Efficient Disulfide Bond Determination Using Multiple Methods
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Comparison with other methods
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1. Methods Selection and Data Entry
2. Reliability Assignment
3. Combination Strategies
4. Global Connectivity
"Point the mouse over each input field or output result to read its description. For more details, please visit our
HELP
section."
Click to select/deselect
the S-S connectivity determination methods to be used:
Enter protein's
FASTA
sequence:
Region where S-S bonds are not expected to occur:
From:
to
(optional)
MS/MS Details
(only if MS/MS method was selected)
Upload a MS/MS data file:
Choose protease used in digestion:
Trypsin
Chymotrypsin
Trypsin + Chymotrypsin
Glu-C
Multi-ion types selection:
a
ao
a*
b
b*
bo
c
x
y
y*
yo
z
Number of missing cleavages:
Optional
0
1
2
3
Initial Matching threshold:
(default: +-1.0)
Input for Custom method
(only if Custom method was selected)
Enter the disulfide bonds found and their respective scores separated by commas:
(i.e.:142-292,0.47,156-356,0.53)
Input for Custom method
(only if Custom method was selected)
Enter the disulfide bonds found and their respective scores separated by commas:
(i.e.:142-292,0.47,156-356,0.53)
